Yeast-display Technology for the Rapid Development of Effective Antiviral and Anti-cancer Vaccines

I discuss a paradigm-shifting influenza vaccine technology which presents H5N1 hemagglutinin (HA) on the surface of yeast. The HA surface-presented yeast can be used as influenza vaccines to elicit both humoral and cell-mediated immunity in mice. The HI titer of antisera reached up to 128 in vaccinated mice. A high level of H5N1 HA-specific IgG1 and IgG2a antibody production was detected after boost immunization. Furthermore, we demonstrated that the yeast surface-displayed HA preserves its antigenic sites. It preferentially binds to both avian- and human-type receptors. The vaccine exhibited high cross-reactivity to both homologous and heterologous H5N1 viruses. A high level production of anti-HA antibodies was detected in the mice five months after vaccination. The yeast vaccine offered complete protection of mice from lethal H5N1 virus challenge. The same technology combined with CRISPR applied to CD47+/+ tumor cells can generate customized and highly effective antitumor vaccines. This new technology allows for rapid and large-scale production of vaccines. If you found this video useful, please check out our other videos on computational modeling, infection and immunology: https://youtube.com/playlist?list=PLiEtieOeWbMKh9VcQoinSwODcSZKMTGat Please consider joining our IMAG/MSM WG on Multiscale Modeling and Viral Pandemics: https://www.imagwiki.nibib.nih.gov/content/msm-viral-pandemics-meetings Please also consider joining the Global Alliance for Immune Prediction and Intervention: http://glimprint.org/