Effect of Spatial Inhomogeneities on the Membrane Surface on Receptor Dimerization and Signal Initiation in Pre-B Cells

We employed spatial stochastic simulations to examine how plasma membrane inhomogeneities impact receptor dimerization and phosphorylation, in the context of tonic signaling by pre-B cell receptors. This approach was developed and validated using data from single-particle tracking experiments. I will focus on the computational methodologies applied in our study. Specifically, we utilized a rule-based framework to capture the complex interactions and potential oligomer formations of pre-B cell receptors, Lyn, and Syk. Our simulations were spatially resolved, integrating domain shapes from experimental data to accurately simulate molecular aggregate diffusion and collisions. This methodology enabled us to reveal key insights into receptor behavior, underscoring the importance of membrane domain organization in signaling processes. For more information see: https://ieeexplore.ieee.org/document/9765396 *Contents* 00:00 - Introduction 07:11 - Effect of Spatial Inhomogeneities on the Membrane Surface on Receptor Dimerization and Signal Initiation in Pre-B Cells 54:43 - Questions and Discussions Moderator: James A. Glazier, PhD, Indiana University, Bloomington If you found this video useful, please check out our other videos on computational modeling, infection and immunology: https://youtube.com/playlist?list=PLiEtieOeWbMKh9VcQoinSwODcSZKMTGat Please consider joining our IMAG/MSM WG on Multiscale Modeling and Viral Pandemics: https://www.imagwiki.nibib.nih.gov/content/msm-viral-pandemics-meetings Please also consider joining the Global Alliance for Immune Prediction and Intervention: http://glimprint.org/